Product Description

PRPF18 Polyclonal Antibody

Catalogue Numbers: BS5866-50, BS5866-100

Sizes: 50µl, 100µl

Alternative Name: Pre-mRNA-splicing factor 18; PRP18 homolog; hPRP18; PRP18; HPRP18

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99633

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: PRPF18 (pre-mRNA-splicing factor 18), also known as HPRP18, is a 342 amino acid protein that localizes to nuclear speckles and plays a role in the second step of pre-mRNA splicing. A member of the PRP18 family, PRPF18 contains seven WD repeats and exists as two alternatively spliced isoforms which are encoded by a gene located on human chromosome 10p13. Chromosome 10 contains over 800 genes and 135 million nucleotides. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. Other chromosome 10 associated disorders include Cockayne syndrome, tetrahydrobiopterin deficiency and trisomy 10.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PRPF18 polyclonal antibody detects endogenous levels of PRPF18 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRPF18 polyclonal antibody at 1:500 dilution
Lane 1: HEK29T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 223-268 of Human PRPF18.

Conjugate: Unconjugated

Modification: Unmodified