Product Description

PRPF8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91119-50, BS91119-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6P2Q9(Human) Q99PV0(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: PRPF8 polyclonal antibody detects endogenous levels of PRPF8 protein.

Bioworld Molecular Weight: 274 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PRPF8 on SiHa (1) and A549 (2) cell lysate using anti-PRPF8 antibody at 1/1,000 dilution., ICC staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PRPF8, PRPC8, 220 kDa U5 snRNP specific protein, 220 kDa U5 snRNP-specific protein, Apoptosis regulated protein 1, Apoptosis regulated protein 2, HPRP8, p220, Pre mRNA processing factor 8, Pre mRNA-processing factor 8, S. cerevisiae, homolog of, Pre-mRNA-processing-splicing factor 8, Precursor mRNA processing protein, PRP8, PRP8 homolog, PRP8 pre mRNA processing factor 8 homolog, PRP8, PRPC8, Prpf8, Retinitis pigmentosa 13 (autosomal dominant), RP13, SNRNP220, Splicing factor Prp8, U5 snRNP specific protein, U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p,

Immunogen: Recombinant protein within 1–200 of human PRPF8.

Conjugate: Unconjugated

Modification: Unmodified