PRSS8 Polyclonal Antibody
Catalogue Numbers: BS60585-50, BS60585-100
Sizes: 50µl, 100µl
Alternative Name: Prostasin; Channel-activating protease 1; CAP1; Serine protease 8; PRSS8
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16651
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Prostasin, a serine protease first identified in prostate tissue, activates epithelial sodium channels in a variety of tissues. Though typically a membrane-anchored protein, free prostasin is also found in physiologic fluids and tissue culture media, indicating a mechanism for secretion from the cells as well. Aprotinin and other protease inhibitors suppress the channel-activating capacity of prostasin, while aldosterone increases prostasin expression and stimulates sodium uptake. In addition, DNA methylation negatively correlates with prostasin expression in cancer cells, while enforced reexpression decreases invasiveness as well as metastatic potential, implying that prostasin activity reflects epithelial cell physiology.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PRSS8 polyclonal antibody detects endogenous levels of PRSS8 protein.
Bioworld Molecular Weight: ~ 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of PRSS8 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human PRSS8
Conjugate: Unconjugated
Modification: Unmodified