Prune homolog 2/C9orf65 Polyclonal Antibody-BS65841

Prune homolog 2/C9orf65 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65841-50, BS65841-100

Swiss-Prot: Q8WUY3

Host: Rabbit

Prune homolog 2/C9orf65 Polyclonal Antibody-BS65841

Storage and Stability: Store at 4°C short term. Aliquot and store at -14°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Purification and Purity: affinity purified by Protein A

Specificity: Prune homolog 2/C9orf65 Polyclonal Antibody detects endogenous levels of Prune homolog 2/C9orf65 protein.

Bioworld Molecular Weight: ~341 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated 、 at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat brain tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: A214N16 3; BCH motif containing molecule at the carboxyl terminal region 1; BMCC1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIPXL; C9orf65; chromosome 9 open reading frame 65; prune homolog 2 (Drosophila); Prune2.

Immunogen: KLH conjugated synthetic peptide derived from human Prune homolog 2/C9orf56:2801-2900/3088

Modification: Unmodified

Conjugate: Unconjugated