Product Description

PRX III (K93) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3468-50, BS3468-100

Sizes: 50µl, 100µl

Swiss-Prot: P30048

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PRX III (K93) polyclonal antibody detects endogenous levels of PRX III protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRX III (K93) polyclonal antibody at 1:500 dilution
Lane 1: 786-O whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: HCT116 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: The Heart tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of PRX III (K93) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 25, 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; PRDX3; AOP1

Immunogen: Synthetic peptide, corresponding to amino acids 60-114 of Human PRX III.

Conjugate: Unconjugated

Modification: Unmodified