Product Description

PSCA (V71) Polyclonal Antibody

Catalogue Numbers: BS1301-50, BS1301-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43653

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol GPI)-anchored cell surface antigens. The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta. The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers. Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PSCA (V71) polyclonal antibody detects endogenous levels of Prostate Stem Cell Antigen protein.

Extra Notes: Western blot (WB) analysis of PSCA (V71) pAb at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:CT26 whole cell lysate(40ug)
Lane4:PC12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PSCA; PRO232; Prostate stem cell antigen; UNQ206

Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human PSCA.

Conjugate: Unconjugated

Modification: Unmodified