Product Description

PSMB9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91128-50, BS91128-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28065(Human) P28076(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC FC IP,

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Background: The eukaryotic multicatalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7-E1 and E2, in several tissues.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PSMB9 polyclonal antibody detects endogenous levels of PSMB9 protein.

Bioworld Molecular Weight: 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse spleen tissue
Lane 2: U937
Lane 2: Mouse colon tissue, Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Proteasome 20S LMP2 antibody. Counter stained with hematoxylin.

Alternative Name: PSMB9, LMP2, PSMB6i, RING12, Beta1i, Large multifunctional peptidase 2, Large multifunctional protease 2, LMP 2, LMP2, Low molecular mass protein 2, Macropain chain 7, MGC70470, Multicatalytic endopeptidase complex chain 7, OTTHUMP00000062982, Proteasome (prosome macropain) subunit beta type 9, proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2), Proteasome beta 9 subunit, Proteasome catalytic subunit 1i, Proteasome chain 7, Proteasome related gene 2, Proteasome subunit beta 6i, Proteasome subunit beta type 9, Proteasome subunit beta type-9, Proteasome subunit beta-1i, PSB9, PSMB 9, PSMB6i, PSMB9, Really interesting new gene 12 protein, RING 12, RING12, RING12 protein,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified