Product Description

PSMC2 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66975-50, MB66975-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of two sub-complexes, the 20S catalytic core particle (CP) and the 19S/PA700 regμlatory particle (RP) that can cap either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β1-7) that contain three catalytic β-subunits and are flanked on either side by two heteroheptameric α-rings (α1-7). The RP includes a base and a lid, each having mμltiple subunits. The base, in part, is composed of a heterohexameric ring of ATPase subunits belonging to the AAA (ATPases Associated with diverse cellμlar Activities) family. The ATPase subunits function to unfold the substrate and open the gate formed by the α-subunits, thus exposing the unfolded substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that function in recruitment of ubiquitinated substrates and modification of ubiquitin chain topology . Other modμlators of proteasome activity, such as PA28/11S REG, can also bind to the end of the 20S CP and activate it . The base of the eukaryotic proteasome 19S/PA700 RP contains six AAA-ATPase subunits (PSMC1-PSMC6) that bind directly to the 20S CP α-ring. These 19S RP ATPases are thought to assemble into a heterohexameric, pore-like structure that forms part of the substrate translocation channel. Energy derived from ATP hydrolysis by the AAA-ATPases is utilized for substrate unfolding and translocation, which is required for degradation of ubiquitinated folded proteins within the central chamber of the 20S CP formed by β-subunits . PSMC2 (RPT1, MSS1) is a AAA-ATPase subunit of the 19S/PA700 RP. Research studies have shown that PSMC2 is associated with several components of the basal transcriptional machinery suggesting that PSMC2, in addition to participating in proteasome-dependent degradation of proteins, may also play a role in gene transcription. More recently, it has been shown that numerous human cancer cell lines have reduced PSMC2 expression resμlting from loss of PSMC2 copy number loss and display a strict threshold requirement for PSMC2 levels in order to sustain a proliferative advantage .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: MSS1, 26S protease regμlatory subunit 7, 26S proteasome AAA-ATPase subunit RPT1, Proteasome 26S subunit ATPase 2, Protein MSS1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human PSMC2. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of PSMC2 protein.

Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P35998

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only