Product Description

PSMC3 (V306) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3778-50, BS3778-100

Sizes: 50µl, 100µl

Swiss-Prot: P17980

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PSMC3 (V306) polyclonal antibody detects endogenous levels of PSMC3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PSMC3 (V306) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (20ug)
Lane 2: HEK293T whole cell lysate (20ug)
Lane 3: The Uterus tissue lysate of Rat (20ug)
Lane 4: The Kidney tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of PSMC3 (V306) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 49 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Human immunodeficiency virus tat transactivator binding protein 1; MGC8487; Proteasome (prosome macropain) 26S subunit ATPase 3; Proteasome 26S ATPase subunit 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; PRS6A; PSMC 3; PSMC3; Tat binding protein 1; TAT-binding protein 1; TBP-1; TBP1;

Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human PSMC3.

Conjugate: Unconjugated

Modification: Unmodified