Product Description

PSMC6 Polyclonal Antibody

Catalogue Numbers: BS5869-50, BS5869-100

Sizes: 50µl, 100µl

Alternative Name: 26S protease regulatory subunit 10B; 26S proteasome AAA-ATPase subunit RPT4; Proteasome 26S subunit ATPase 6; Proteasome subunit p42; SUG2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62333

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. PSMC6 (proteasome (prosome, macropain) 26S subunit, ATPase 6), also known as P44, p42, SUG2, S10B or CADP44, is a regulatory component of the 26S proteasome. More specifically, PSMC6 is one of the six ATPase subunits of the 19S regulator base. It contains a leucine zipper motif and an AAA (ATPase associated with diverse cellular activities) domain and belongs to the AAA ATPase family of chaperone-like ATPases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PSMC6 polyclonal antibody detects endogenous levels of PSMC6 protein.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PSMC6 pAb at 1:1000 dilution
Lane 1: HCT116 whole cell lysate(40ug)
Lane 2: The Testis tissue lysate of Rat(40ug)
Lane 3: The Testis tissue lysate of Mouse(40ug)
Lane 4: Hela whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 107-152 of Human PSMC6.

Conjugate: Unconjugated

Modification: Unmodified