PTBP1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9458-50, MB9458-100
Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P26599(Human) Q00438(Rat)
Host: Mouse
Reactivity: Human, Rat
Applications: WB, IHC ICC FC
All Applications: WB:1:5,000-1:10,000
IHC:1:50-1:100
ICC:1:50-1:100
FC:1:50-1:100
Background: Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
Purification and Purity: ProG affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: PTBP1 monoclonal antibody detects endogenous levels of PTBP1 protein.
Bioworld Molecular Weight: 57 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A431
Lane 3: K562, ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PTB, hnRNP I, PTBP1, PTB, 57 kDa RNA binding protein PPTB 1, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, Heterogeneous Nuclear Ribonucleoprotein Polypeptide I, hnRNP I, HNRNP-I, HNRNPI, HNRPI, MGC10830, MGC8461, Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), Polypyrimidine Tract Binding Protein 1, Polypyrimidine tract binding protein, Polypyrimidine tract-binding protein 1, pPTB, PTB 1, PTB 2, PTB 3, PTB 4, PTB, PTB T, PTB1, PTB2, PTB3, PTB4, PTBP 1, PTBP1, PTBP1, PTBT, RNA Binding Protein,
Immunogen: Recombinant protein within human PTBP1 aa 320-550.
Conjugate: Unconjugated
Modification: Unmodified