Product Description

PTS Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS8117-50, BS8117-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The tetrahydrobiopterin (BH4) cofactor is essential for hepatic hydroxylase, which is involved in phenylalanine degradation and catecholamine and serotonin biosynthesis. BH4 is also an essential and limiting cofactor for all types of nitric oxide synthase. BH4 deficiency resμlts in hyperphenylalaninemia and monoamine neurotransmitter depletion and is most commonly due to autosomal recessive mutation in 6-pyruvoyltetrahydropterin synthase (PTPS), the second enzyme for BH4 biosynthesis. The active site of PTPS consists of the pterin-anchoring Glu A107 neighbored by two catalytic motifs: a Zn(II) binding site and an inter-subunit catalytic triad formed by Cys A42, Asp B88 and His B89. The active site of PTPS undergoes a Zn and Mg-dependent reaction that includes a triphosphate elimination, a stereospecific reduction and the oxidation of both side hydroxyl groups. The catalytic triad of PTPS is involved in the deprotonation of the side-chain carbons of substrates. In addition, Ser 19 of human PTPS may be a substrate for cGMP-dependent protein kinase type II phosphorylation in vivo, which is essential for normal activity of PTPS.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: 6-pyruvoyl tetrahydrobiopterin synthase, PTP synthase, PTPS, PTS

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human PTS.

Dilution: WB: 1:500~1:2000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PTS polyclonal antibody detects endogenous levels of PTS protein.

Molecular Weight: ~ 16 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q03393

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only