Product Description

PTTG1/2/3 (L149) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1909-50, BS1909-100

Sizes: 50µl, 100µl

Swiss-Prot: O95997/Q9NZH5/Q9NZH4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: PTTG contains a basic amino-terminal domain and an acidic carboxy-terminal domain, which acts as a transactivation domain when fused to a heterologous DNA binding domain. Human PTTG is overexpressed in Jurkat and is also detected in human thymus, testis and placenta. PTTG is mainly expressed in the cytoplasm and is also partially localized to the nucleus. Vertebrate PTTG regulates the separin Esp1, which promotes chromatid separation, to overcome the cohesive forces that hold sister chromatids together. This regulatory function of PTTG suggests that defective regulation of cohesion may contribute to cancer by promoting chromosome instability. Although vertebrate PTTG shares cell-cycle functions with its yeast securin counterparts Pds1p and Cut2, none share sequence homology.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PTTG1/2/3 (L149)pAb detects endogenous levels of PTTG1/2/3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PTTG1/2/3 (L149) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: H1792 whole cell lysate (40ug)
Lane 3: A375 whole cell lysate (20ug)
Lane 4: AML-12 whole cell lysate (40ug)
Lane 5: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PTTG1/2/3 (L149) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG; PTTG1; EAP1; PTTG; TUTR1; Securin-2; Securin2; Pituitary tumor-transforming gene 2 protein; PTTG2; Putative pituitary tumor-transforming gene 3 protein; hPTTG3; Securin-3; rcPTTG1; PTTG3P; PTTG3; Securin3;

Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human PTTG1.

Conjugate: Unconjugated

Modification: Unmodified