Product Description

PU.1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66911-50, MB66911-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box . PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes. The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regμlation of differentiation versus stem cell proliferation . In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobμlin κ 3' enhancer . Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resμlting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1. GATA1 binding blocks PU.1 activity during erythroid cell development. Overexpression of PU.1 resμlting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: Transcription factor PU.1, 31 kDa-transforming protein

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human PU.1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of PU.1 protein.

Molecular Weight: ~ 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17947

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only