Product Description

PU.1 (R212) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2649-50, BS2649-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Ets transcription factor family (Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granμlocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumμlates through T lineage commitment and then is dramatically absent following the β selection checkpoint.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: SPI1, PU.1, SFPI1, Transcription factor PU.1, 31 kDa-transforming protein, SPI1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human PU.1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PU.1 (R212) polyclonal antibody detects endogenous levels of PU.1 protein.

Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17947

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only