PU.1/Spi1 Polyclonal Antibody-BS91136

PU.1/Spi1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91136-50, BS91136-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17947(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC FC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Background: The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PU.1/Spi1 polyclonal antibody detects endogenous levels of PU.1/Spi1 protein.

Bioworld Molecular Weight: 31 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.

Alternative Name: SPI1, transcription factor spi1, 31 kDa Transforming Protein, 31 kDa-transforming protein, cb1086, Hematopoietic transcription factor PU.1, OF, oncogene spi1, PU.1, SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of, SFPI1, si:by184l24.2, SPI 1, SPI 1 proto oncogene, SPI A, Spi1, SPI1, Spleen focus forming virus (SFFV) proviral integration oncogene spi1, Spleen focus forming virus proviral integration oncogene spi1, Transcription factor PU.1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified