Product Description

PUMA Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11483-50, MB11483-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9BXH1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 21 kDa; Observed MW: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of PUMA in 293T using PUMA antibody, and DAPI., Western blot analysis of PUMA in K562 lysates using PUMA antibody., Western blot analysis of PUMA in Jurkat, THP-1, C6, 3T3, Hela lysates using PUMA antibody, Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PUMA antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: BBC3; PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis

Immunogen: A synthetic peptide of human PUMA

Conjugate: Unconjugated

Modification: Unmodified