Puromycin Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-92336-60, E-AB-92336-120, E-AB-92336-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Puromycin
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB
Isotype: IgG
Clonality: Polyclonal
Background: Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.
Concentration: 1mg/mL
Immunogen: Chemical compounds corresponding to Puromycin
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Observed MW: 10-250KDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only