Product Description

PXR Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7284-50, BS7284-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75469

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 50KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PXR antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of C6 cells using PXR Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 600ug extracts of Mouse stomach cells using 3ug PXR antibody. Western blot was performed from the immunoprecipitate using PXR antibody at a dilution of 1:500.

Alternative Name: NR1I2; BXR; ONR1; PAR; PAR1; PAR2; PARq; PRR; PXR; SAR; SXR

Immunogen: Recombinant fusion protein of human PXR(NP_003880.3).

Conjugate: Unconjugated

Modification: Unmodified