Product Description

QSK Polyclonal Antibody

Catalogue Numbers: BS62472-50, BS62472-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q9Y2K2

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine (Ser/Thr) protein kinases. The salt-inducible kinases (SIKs) are a family of related serine-threonine kinases and are key enzymes that modulate important processes such as steroid hormone biosynthesis and Insulin signaling in adipocytes. QSK, also known as L19 or SIK3 (salt-inducible kinase 3), is a 1,263 amino acid cytoplasmic protein belonging to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and the AMPK subfamily. Ubiquitously expressed, QSK consists of one protein kinase domain and a UBA domain. QSK is activated by 14-3-3 ζ and utilizes magnesium as a cofactor. QSK exists as three alternatively spliced isoforms.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Specificity: QSK polyclonal antibody detects endogenous levels of QSK protein.

Extra Notes: Western blot (WB) analysis of QSK polyclonal antibody at 1:500 dilution
LaneA:L02 whole cell lysate
LaneB:MCF-7 whole cell lysate
LaneC:A549 whole cell lysate
LaneD:CT-26 whole cell lysate
LaneE:PC12 whole cell lysate

Bioworld Molecular Weight: ~ 140 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Serine/threonine-protein kinase SIK3; Salt-inducible kinase 3; SIK-3; Serine/threonine-protein kinase QSK; SIK3; KIAA0999; QSK; L19

Immunogen: Synthetic peptide, corresponding to Human QSK.

Conjugate: Unconjugated

Modification: Unmodified