QuicKey Human Cys-C (Cystatin C) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-TSEL-H0008-24, E-TSEL-H0008-48, E-TSEL-H0008-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: Cys-C
UNIProt ID: P01034
Target Synonyms: CST3; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin;
Research Areas: Immunology,Metabolism,Cardiovascular
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human Cys-C concentrations in Serum, Plasma, Urine, Saliva.
Tested Sample Types: Serum, Plasma, Urine, Saliva
Type: Sandwich
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivity: 0.04 ng/mL
Detection Range: 0.31-20 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. You can calculate the concentration of Human Cys-C in the samples by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag