RAB35 Polyclonal Antibody-BS5877

RAB35 Polyclonal Antibody

Catalogue Numbers: BS5877-50, BS5877-100

Sizes: 50µl, 100µl

Alternative Name: Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C; RAB1C; RAY

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15286

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab 35, also known as Rab 1C or RAY, is a 201 amino acid lipidanchored protein that is thought to function as a GTPase, playing a role in exocytotic and endocytotic pathways and functioning as an important factor in cytokinesis. Rab 35 is expressed ubiquitously and is encoded by a gene that maps to human chromosome 12, which houses over 1,100 genes and comprises approximately 4.5% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RAB35 polyclonal antibody detects endogenous levels of RAB35 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RAB35 pAb at 1:500 dilution
Lane 1: Hela whole cell lysate(40ug)
Lane 2: L02 whole cell lysate(40ug)
Lane 3: The Testis tissue lysate of Rat(40ug)
Lane 4: The Testis tissue lysate of Mouse(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human RAB35.

Conjugate: Unconjugated

Modification: Unmodified