RAB3A Monoclonal Antibody-MB66919

RAB3A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66919-50, MB66919-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P20336

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, FC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Background: The Rab family of proteins includes small, monomeric GTPases essential for regulating intracellular vesicle trafficking. Members of the Rab3 subfamily, including Rab3A-3D, are involved in the exocytosis of neurotransmitters and hormones. Rab3A is primarily expressed in neurons, neuroendocrine cells , and in human pancreatic 尾 cells . By acting as a molecular switch between active GTP-bound Rab3A and the inactive GDP-bound form, Rab3A inhibits synaptic vesicle and chromaffin granule secretion during late membrane release . Loss-of-function studies suggest Rab3A is involved in controlling synaptic vesicle targeting and docking at the active zone . Through binding to its direct effector Rabphillin, Rab3A also orchestrates the coupling between synaptic vesicle exocytosis and endocytosis

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of RAB3A protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RAB3A expression in mouse brain (A), rat brain (B) whole cell lysates., Immunofluorescent analysis of RAB3A staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Alternative Name: Ras-related protein Rab-3A

Immunogen: Recombinant fusion protein of human RAB3A. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified