RAB7 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91141-50, BS91141-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P51149(Human) P51150(Mouse) P09527(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: RAB7 polyclonal antibody detects endogenous levels of RAB7 protein.
Bioworld Molecular Weight: 23 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: C2C12, ICC staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: RAB7A, CMT2B, PRO2706, PSN, RAB7, member RAS oncogene family, RAB7A, RAB7A, member RAS oncogene family, RAB7A, Ras associated protein RAB7, Ras related protein Rab7, Ras related protein Rab7a, Ras-related protein Rab-7a,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified