Product Description

Rabphilin-3A (P231) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1590-50, BS1590-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9Y2J0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B,and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rabphilin 3A (P231) polyclonal antibody detects endogenous levels of Rabphilin 3A protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rabphilin-3A (P231) polyclonal antibody at 1:500 dilution
Lane 1: THP-1 whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of Rabphilin-3A (P231) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Rabphilin-3A; Exophilin-1; Exophilin 1; Exophilin1; Rph3a

Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human Rabphilin-3A.

Conjugate: Unconjugated

Modification: Unmodified