Rad17 Polyclonal Antibody - BT-AP07600

Rad17 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP07600-20, BT-AP07600-50, BT-AP07600-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by RAD17 (RAD17 checkpoint clamp loader component) is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: RAD17

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: Rad17 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 77055

UniProt Accession: Human: O75943; Mouse: Q6NXW6

Synonyms: RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog

GeneID: Human: 5884; Mouse: 19356

Research Use Only