Rad21 (D548) Polyclonal Antibody-BS2422

Rad21 (D548) Polyclonal Antibody

Catalogue Numbers: BS2422-50, BS2422-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60216

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis. The cleavage product migrates to the cytoplasm and is involved in early events in the apoptotic pathway and it amplifiies the cell death signal in a positive-feedback manner.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rad21 (D548) polyclonal antibody detects endogenous levels of Rad21 protein.

Extra Notes: Western blot (WB) analysis of Rad21 (D548) pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:3T3-L1 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; RAD21; HR21; KIAA0078; NXP1

Immunogen: Synthetic peptide, corresponding to amino acids 521-570 of Human Rad21.

Conjugate: Unconjugated

Modification: Unmodified