Product Description

RAD21 Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66451-50, MB66451-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase . RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms, however, cohesin remains bound to centromeres until anaphase. RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis. RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resμlting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis. In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regμlation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage.

Category: Primary Antibody

Reactivity: Human, Hamster

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: HR21, KIAA0078, NXP1, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human Rad21

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of RAD21 protein.

Molecular Weight: ~ 110 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60216

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only