Rad23B (5H1) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0186-50, MB0186-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P54727
Host: Mouse
Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, ICC IHC
All Applications: WB: 1:1000
IHC/ICC: 1:50~200
Background: The RAD23 genes (RAD23A and RAD23B), which encode the human Rad23 proteins, are crucial for excision-repair of UV-damaged DNA. RAD23 genes resemble the other DNA repair genes, RAD2, RAD6, RAD7, RAD18 and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Rad23 is a nuclear protein containing an ubiquitin-like domain required for biological functions. The protein, which is highly conserved, is involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. The C-terminal ubiquitin-associated domain of Rad23 is evolutionarily conserved from yeast to humans. Rad23 may also act as a regulator for the activity of the 26S Proteasome.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Rad23B and does not cross-react with related proteins.
Bioworld Molecular Weight: Predicted band size: 55KDa
Observed band size: 55KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot detection of Rad23B in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using Rad23B mouse mAb (1:1000 diluted)., Immunohistochemical analysis of paraffin-embedded Prostate Cancer using Rad23B mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0)., Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Rad23B antibody (dilution 1:100).
Alternative Name: UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58; RAD23B
Immunogen: Purified recombinant human Rad23B protein fragments expressed in E.coli.
Conjugate: Unconjugated
Modification: Unmodified