RAD23B Monoclonal Antibody
Catalogue Numbers: MB67024-50, MB67024-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P54727
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: The yeast nucleotide excision repair (NER) radiation sensitive protein 23 (rad23) and its human homologs Rad23A (hHR23A) and Rad23B (hHR23B) are critical components of the cellular machinery that recognize DNA lesions and serve as receptors that target ubiquitinated substrates to the proteasome for degradation .
The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex . In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities . Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome . Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions , as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome . Research studies have shown that targeted deletion of the murine Rad23b locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development .
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of RAD23B protein.
Extra Notes: Western blot analysis of RAD23B expression in Jurkat (A), A549 (B), HUVEC (C), NIH3T3 (D), mouse brain (E), rat brain (F) whole cell lysates.
Bioworld Molecular Weight: ~ 53 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human RAD23B. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified