Product Description

Rad51B (G226) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2542-50, BS2542-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15315

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The DNA repair proteins Rad50, Rad51A-D, Rad52 and Rad54 A and B are key components of the double-strand break repair pathway (DSBR). The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51B is a widely expressed protein that is involved in the repair of double-stranded DNA breaks that can arise during DNA replication. It can form a dimer with Rad51C and this complex shows single-stranded DNAdependent ATPase activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rad51B (G226) polyclonal antibody detects endogenous levels of Rad51B protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human lung carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; Rad51B; RAD51-like protein 1; RAD51B; RAD51L1; REC2

Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human Rad51B.

Conjugate: Unconjugated

Modification: Unmodified