Product Description

Rad51C (E185) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2421-50, BS2421-100

Sizes: 50µl, 100µl

Swiss-Prot: O43502

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51C binds single- and double-stranded DNA and preferentially binds 3'-end-tailed double-stranded DNA. Rad51C is expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rad51C (E185) polyclonal antibody detects endogenous levels of Rad51C protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rad51C (E185) pAb at 1:500 dilution
Lane 1: H9C2 whole cell lysate (40ug)
Lane 2: The Testis tissue lysate of Mouse (40ug)
Lane 3: MCF-7 whole cell lysate (40ug)
Lane 4: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rad51C (E185) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2

Immunogen: Synthetic peptide, corresponding to amino acids 161-210 of Human Rad51C.

Conjugate: Unconjugated

Modification: Unmodified