Product Description

RAD9A Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66553-50, MB66553-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA damage resμlting from genotoxic stress activates cellμlar checkpoints that prevent or delay cell division until either damaged DNA is repaired or the cell follows an apoptotic pathway. The Rad9 homolog A (Rad9A, Rad9) protein is part of a checkpoint protein complex that acts as an early sensor of DNA damage. Together with the Hus1 and Rad1 checkpoint proteins, Rad9 forms a heterotrimeric 9-1-1 complex with a ring structure similar to the processivity factor PCNA. The 9-1-1 complex induces mμltiple signaling pathways, including the ATM and ATR-activated DNA repair pathways. A functional 9-1-1 complex is required for ATR-dependent S phase checkpoint signaling. The 9-1-1 complex interacts with DNA topoisomerase 2-binding protein 1 (TopBP1) in response to DNA damage, activating ATR and causing signal amplification through further recruitment of TopBP1. The 9-1-1 complex interacts with DNA mismatch repair proteins MSH2, MSH3, and MSH6 to play a role in mismatch repair. During an error-free DNA damage tolerance process, the 9-1-1 complex cooperates with polyubiquitinated PCNA and Exo1 nuclease to support switching of the replicative polymerase to the undamaged template. Research studies indicate that the two Rad9 paralogues (Rad9A and Rad9B) can both functionally complement one another and display distinct biological functions. Specifically, Rad9B senses nucleolar stress and causes a delay in the cell cycle at G1/S phase.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Applications: WB, IP

Alternate Names: RAD9A, Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human RAD9A protein fragments expressed in E.coli

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of RAD9A protein.

Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q99638

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only