Radixin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91147-50, BS91147-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35241(Human) P26043(Mouse) Unigene:224619(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Radixin polyclonal antibody detects endogenous levels of Radixin protein.
Bioworld Molecular Weight: 80 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: HepG2, ICC staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: RDX, CB567, CG12537, DFNB24, ESP10, Hh-induced MATH and BTB domain-containing protein, HIB, Moesin-B, Protein roadkill, RADI, Radixin, RDX,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified