Product Description

Raf-1 (phospho-S259) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4163-50, BS4163-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellμlar homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregμlated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regμlatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecμles, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Raf-1 around the phosphorylation site of Serine 259.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Raf1 (S259) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at Ser259

Molecular Weight: ~ 73 kDa

Pathway: Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, hippo Signaling, MAPK Erk in Growth and Differentiation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04049

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only