Product Description

Raf-B (F594) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0422-50, AP0422-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P15056

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Raf-B (F594) polyclonal antibody detects endogenous levels of Raf-B protein.

Bioworld Molecular Weight: ~ 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Raf-B (F594) polyclonal antibody at 1:1000 dilution
Lane 1: K562 cell membrane lysate (24ug)
Lane 2: The Lung tissue lysate of Mouse (30ug)
Lane 3: BV2 whole cell lysate (30ug)
Lane 4: PC12 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using Raf-B (F594) pAb at dilution of 1:200.

Alternative Name: Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1

Immunogen: Synthetic peptide, corresponding to Human Raf-B.

Conjugate: Unconjugated

Modification: Unmodified