Product Description

Raf-B (Y198) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1932-50, BS1932-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellμlar homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregμlated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regμlatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecμles, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: IHC, IF

Alternate Names: Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 170-220 of Human Raf-B.

Dilution: IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Raf-B (Y198) polyclonal antibody detects endogenous levels of Raf-B protein.

Molecular Weight: ~ 86 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15056

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only