RAF1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-60020-60, E-AB-60020-120, E-AB-60020-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: RAF1
Target Synonym: CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1
Research Areas: Cancer, Cell Biology, Metabolism, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P04049
Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human RAF1 (NP_002871.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Calculated MW: 73kDa/75kDa
Observed MW: 73kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only