RAGE Polyclonal Antibody-BS91150

RAGE Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91150-50, BS91150-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15109(Human) Q62151(Mouse) Q63495(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: RAGE polyclonal antibody detects endogenous levels of RAGE protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution., ICC staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: AGER, Advanced glycosylation end product-specific receptor, Ager, MGC2235, RAGE, Receptor for advanced glycosylation end products,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified