Product Description

Rak (N103) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1996-50, BS1996-100

Sizes: 50µl, 100µl

Swiss-Prot: P42685

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Src is the human homolog of the v-src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic nonreceptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Frk (Rak) is an epithelial tissue-specific kinase. The human Rak gene maps to chromosome 6q21-q22.3 and encodes a 505 amino acid protein.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rak (N103) polyclonal antibody detects endogenous levels of Rak protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rak (N103) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: CT26 whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rak (N103) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; RAK; FRK

Immunogen: Synthetic peptide, corresponding to amino acids 80-120 of Human Rak.

Conjugate: Unconjugated

Modification: Unmodified