RAMP1 Polyclonal Antibody
Catalogue Numbers: BS60515-50, BS60515-100
Sizes: 50µl, 100µl
Alternative Name: Receptor activity-modifying protein 1; Calcitonin-receptor-like receptor activity-modifying protein 1; CRLR activity-modifying protein 1; RAMP1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60894
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Receptor activity-modifying proteins (RAMPs) are transmembrane accessory proteins that influence the pharmacological profiles of the calcitonin receptor-like receptors (CRLR). RAMPs associate with CRLR in the endoplasmic reticulum and facilitate the glycosylation and transport of CRLR to the cell surface, where the mature protein then operates as a receptor for two structurally related vasodilatory peptides, calcitonin-gene-related peptide (CGRP) or adrenomedullin (ADM). RAMP-1 associating with CRLR confers a CGRP receptor, while RAMP-2 and RAMP-3 preferentially induce a responsiveness to ADM. RAMP proteins, including RAMP-1, RAMP-2 and RAMP-3, are structurally similar as they are type I receptors, which have a single extracellular N-terminus and a cytoplasmic C-terminus, and they share approximately 55% sequence similarity. RAMP-1 expression is highest in the uterus, brain and gastrointestinal tract, whereas RAMP-2 and RAMP-3 are highest in lung, breast and fetal tissues.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: RAMP1 polyclonal antibody detects endogenous levels of RAMP1 protein.
Bioworld Molecular Weight: ~ 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of RAMP1 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human RAMP1.
Conjugate: Unconjugated
Modification: Unmodified