Product Description

RANBP1 Polyclonal Antibody

Catalogue Numbers: BS60214-50, BS60214-100

Sizes: 50µl, 100µl

Alternative Name: Ran-specific GTPase-activating protein; Ran-binding protein 1; RanBP1;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43487

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The small Ras-related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The Ran BP2 protein has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RANBP1 polyclonal antibody detects endogenous levels of RANBP1 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RANBP1 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human RANBP1.

Conjugate: Unconjugated

Modification: Unmodified