Product Description

RAPGEF3 Polyclonal Antibody

Catalogue Numbers: BS5713-50, BS5713-100

Sizes: 50µl, 100µl

Alternative Name: Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI; CGEF1; EPAC; EPAC1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95398

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RAPGEF3 polyclonal antibody detects endogenous levels of RAPGEF3 protein.

Bioworld Molecular Weight: ~ 99 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RAPGEF3 polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 799-844 of Human RAPGEF3.

Conjugate: Unconjugated

Modification: Unmodified