RARα Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91155-50, BS91155-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P10276(Human) P11416(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100
Background: Retinoids (RA) are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. RAs activate the retinoic acid receptor (RAR) and retinoid X receptor (RXR) nuclear transcription factor families. Most retinoid forms activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RAR family members, which include RARα, RARβ and RARγ, have a high affinity for all transretinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. The human RARα gene maps to chromosome 17 and is implicated in the chromosomal translocation associated with acute promyelocytic leukemia (APL-M3). Specifically, the RARα gene is fused with the promyelocytic leukemia (PML) gene, which encodes the fusion protein PML/RARα. The PML/RARα fusion protein inhibits PML-dependent apoptotic pathways and halts myeloid differentiation at the promyelocytic stage.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: RARα polyclonal antibody detects endogenous levels of RARα protein.
Bioworld Molecular Weight: 51 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of RAR alpha on different lysates using anti-RAR alpha antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung tissue, ICC staining RAR alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: RAR-alpha, RARA, NR1B1, NR1B1, Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein, Nuclear receptor subfamily 1 group B member 1, Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form, RAR alpha, RAR, RAR-alpha, rara, RARA, RARalpha, RARalpha1, Retinoic acid nuclear receptor alpha variant 1, Retinoic acid nuclear receptor alpha variant 2, Retinoic acid receptor alpha, Retinoic acid receptor alpha polypeptide,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified