RARβ (L359) Polyclonal Antibody-BS1315

RARβ (L359) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1315-50, BS1315-100

Sizes: 50µl, 100µl

Swiss-Prot: P10826

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Retinoids are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are nuclear transcription factors that modulate the effects of retinoids (RA) on gene expression. Most retinoid forms (including all trans RA, 9-cis RA, 4oxo RA and 3,4 dihydro RA) activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RA binds RARs, inducing a change in receptor configuration that allows DNA binding and increased gene transcription from specific genes to occur. RAR family members, which include RARα, RARβ and RARγ, belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. Retinoid receptor expression is tissue specific; the skin expresses RARγ and RXRα.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Retinoic Acid Receptor β (L359) polyclonal antibody detects endogenous levels of Retinoic Acid Receptor β protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RARβ (L359) pAb at 1:500 dilution
Lane 1: SK-OVCAR3 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of RARβ (L359) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 52 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Retinoic acid receptor beta; RAR-beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon; RARB; HAP; NR1B2

Immunogen: Synthetic peptide, corresponding to amino acids 321-370 of Human RARβ.

Conjugate: Unconjugated

Modification: Unmodified