Product Description

Ras (H27) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1309-50, BS1309-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The KRAS gene encodes the human cellμlar homolog of a transforming gene isolated from the Kirsten rat sarcoma virus. The RAS proteins are GDP/GTP-binding proteins that act as intracellμlar signal transducers. The most well-studied members of the RAS (derived from 'RAt Sarcoma' virus) gene family include KRAS, HRAS, and NRAS. These genes encode immunologically related proteins with a molecμlar mass of 21 kD and are homologs of rodent sarcoma virus genes that have transforming abilities. While these wildtype cellμlar proteins in humans play a vital role in normal tissue signaling, including proliferation, differentiation, and senescence, mutated genes are potent oncogenes that play a role in many human cancers.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, HRAS, HRAS1, GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, KRAS, KRAS2, RASK2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human Ras.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ras (H27) polyclonal antibody detects endogenous levels of total K-Ras, H-Ras and N-Ras proteins.

Molecular Weight: ~ 21 kDa

Pathway: Warburg Effect, SAPK JNK Signaling Cascades, Contribution of ECM and Cytoskeletal Factors to EMT, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P01112/P01116/P01111

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only