Product Description

RASA4 Polyclonal Antibody

Catalogue Numbers: BS60215-50, BS60215-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43374

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RASA4 (ras GTPase-activating protein 4) is an 803 amino acid member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogenactivated protein kinase pathway in response to Ca2+. Stimuli that increase intracellular Ca2+ levels result in the translocation of the RASA4 protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth and differentiation. Containing 21 exons and spanning 35 kb of genomic DNA, RASA4 includes one Btk-type zinc finger, two C2 domains, one PH domain and one Ras-GAP domain.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RASA4 polyclonal antibody detects endogenous levels of RASA4 protein.

Extra Notes: Western blot (WB) analysis of RASA4 (F157) pAb at 1:500 dilution
Lane1:3T3-L1 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 85, 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Ras GTPase-activating protein 4; Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2; CAPRI; GAPL; KIAA0538

Immunogen: A synthetic peptide corresponding to residues in Human RASA4.

Conjugate: Unconjugated

Modification: Unmodified