Product Description

RASGRF1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS8617-50, BS8617-100

Swiss-Prot: Q13972

Host: Rabbit

RASGRF1 Polyclonal Antibody-BS8617

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins.Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RASGRF1 polyclonal antibody detects endogenous levels of RASGRF1 protein.

Bioworld Molecular Weight: ~ 145kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extract of Mouse brain, using RASGRF1 antibody., Immunofluorescence analysis of U20S cell using RASGRF1 antibody. Blue: DAPI for nuclear staining.

Alternative Name: Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide; exchange factor CDC25; RASGRF1; CDC25; GNRP; GRF1

Immunogen: Recombinant full length Human RASGRF1.

Modification: Unmodified

Conjugate: Unconjugated