RASGRP2 Polyclonal Antibody-BS76279

RASGRP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76279-50, BS76279-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7LDG7

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using RASGRP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 69kDa

Alternative Name: RASGRP2; CALDAG-GEFI; CDC25L

Immunogen: Recombinant fusion protein of human RASGRP2(NP_722541.1).

Conjugate: Unconjugated

Modification: Unmodified