RASIP1 Polyclonal Antibody
Catalogue Numbers: BS60744-50, BS60744-100
Sizes: 50µl, 100µl
Alternative Name: Ras-interacting protein 1; Rain; RASIP1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q5U651
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: RAIN, also known as RASIP1 (Ras-interacting protein 1), is a 963 amino acid protein that contains one dilute domain and one Ras-associating domain. The RAIN protein is highly expressed in heart and detected at lower levels in placenta and pancreas. RAIN interacts with H-Ras, Rap 1A, Rap 2, R-Ras, Raf-1 and TC 21, as well as with Ras family members that have been activated by GTP binding. RAIN interacts with Ras in a GTP-dependent manner in vitro and in vivo, requiring an intact Ras core effector-binding domain for this interaction, and thus fits the definition of a Ras effector. Unlike other Ras effectors, RAIN localizes to the perinuclear, juxta-Golgi vesicles in intact cells and is recruited to the Golgi by activated Ras. Containing twelve exons, the RAIN gene is conserved in dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 19q13.33.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: RASIP1 polyclonal antibody detects endogenous levels of RASIP1 protein.
Bioworld Molecular Weight: ~ 103 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of RASIP1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human RASIP1
Conjugate: Unconjugated
Modification: Unmodified